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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Ann N Y Acad Sci. 2012 Dec;1273(1):78–84. doi: 10.1111/j.1749-6632.2012.06837.x

Figure 2.

Figure 2

Cartoon depicting method to isolate and analyze phagosomes containing fungal-like particles. Polystyrene beads with fungal-derived carbohydrates covalently attached to the surface are incubated with macrophages. After a defined period, detergent-free lysis releases cellular content including intact phagosomes. Lysates are added to discontinuous sucrose gradients and then are subjected to ultracentrifugation. Phagosomes are easily isolated as a result of the buoyant properties of polystyrene beads and then analyzed by flow cytometry, Western blot, or mass spectrometry of membrane proteins.