Figure 9.

Ll-FIM1 Can Cross-Link F-Actin into Actin Bundles.

(A) Low-speed cosedimentation assays were used to determine the bundling activity of Ll-FIM1. Increasing concentrations of Ll-FIM1 (0.25 to 5 μM) were incubated with F-actin, and samples were subjected to low-speed cosedimentation assays. Gels were analyzed to determine the percentage of actin in the pellets. Error bars indicate mean ± sd (n = 3). P, pellet; S, supernatant.

(B) The ability of Ll-FIM1 to alter actin organization was visualized by Alexa-488 phalloidin staining of F-actin incubated in the absence (a) or presence of Ll-FIM1 (b). Bars = 10 µm.