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. 2012 Nov 19;24(11):4703–4716. doi: 10.1105/tpc.112.104463

Figure 3.

Figure 3.

PUB22 Is Stabilized upon flg22 Treatment.

(A) Expression of recombinant HA-PUB22 or HA-PUB22C13A in Arabidopsis protoplasts 1 h after treatment with flg22 (1 µM), AM114 (20 µM), or equivalent volume of DMSO (−) as indicated. CBB, Coomassie blue.

(B) Expression kinetic of recombinant HA-PUB22 in Arabidopsis protoplasts after treatment with flg22 (1 µM), the proteasomal inhibitor AM114 (20 µM), or equivalent volume of DMSO (−) as indicated. Experiments were repeated at least three times with similar results. Recombinant protein was detected with anti-HA antibody. Equal loading is shown by Coomassie blue staining.