Figure 2.

The Development of Central Vacuoles Is Compromised in keg Mutants.

(A) Confocal microscopy images of vacuoles in the root elongation zone of 5-d light-grown 35S:γ-TIP-mCherry transgenic seedlings. Col-0, Columbia-0.

(B) Confocal microscopy images of vacuoles in hypocotyl of 7-d dark-grown 35S:γ-TIP-mCherry transgenic seedlings.

(C) Confocal microscopy images of vacuoles in cotyledons of 7-d dark-grown 35S:γ-TIP-mCherry transgenic seedlings.

(D) Percentage of cells with fragmented vacuoles in the root elongation zone shown in (A). Data represent means ± sd (n = 30).

(E) Percentage of cells with disrupted vacuoles in etiolated hypocotyl shown in (B). Data represent means ± sd (n = 30).

Bars = 10 μm.

[See online article for color version of this figure.]