Figure 3.

KEG-sYFP Fully Complements keg-1 Mutant Phenotypes and Localizes to the TGN/EE.

(A) Ten-day old seedlings of wild-type plants, 35S:KEG-sYFP transgenic keg-1 plants, and nontransgenic keg-1 plants grown on half-strength MS plates. Col-0, Columbia-0.

(B) KEG genotypes of seedlings shown in (A) analyzed by PCR.

(C) and (D) Three-week-old wild-type plants and 35S:KEG-sYFP transgenic keg-1 plants.

(E) KEG-sYFP protein levels in the plants shown in (C) and (D).

(F) to (H) Cells in the root elongation zone of 35S:KEG-sYFP transgenic keg-1 plants stained with the endocytic tracer FM4-64 for 5 min.

(I) to (N) Protoplasts prepared from 35S:KEG-sYFP transgenic keg-1 plants transfected with 35S:VHA-a1-RFP or 35S:ARA6-mCherry. Images were taken 16 h after transfection.

Bars = 5 μm.