Figure 1.

Translocation of AvrPiz-t into Rice Cells during Rice Blast Infection.

The fungal transformant KV129 expressing AvrPiz-t:mCherry:NLS and putative interfacial matrix protein BAS4:EGFP at 30 h after infection in the sheath cells of rice cultivar YT16 rice is shown as a projection of confocal optical sections over a depth of 4 μm. “Merge” shows bright-field, mCherry (red), and enhanced GFP (green). Arrows indicate BIC, arrowheads indicate rice nucleus, and yellow indicates overlapping mCherry and enhanced GFP fluorescence signals. Pinhole settings were 1 airy unit for enhanced GFP and 12.5 airy units for mCherry. mCherry fluorescence occurred in the BIC and in the nucleus of the invaded rice cell with brighter fluorescence in the presumed nucleolus. Bar = 5 μm.