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. 2012 Nov 30;24(11):4748–4762. doi: 10.1105/tpc.112.105429

Figure 5.

Figure 5.

Degradation of AvrPiz-t in the Presence of APIP6 in N. benthamiana.

FLAG:APIP6 or FLAG:APIP6 H58Y (H58Y) was coexpressed with GFP:AvrPiz-t:HA (without the signal peptide sequence) by agroinfection, and the tissues were harvested 2 DAI. MG132 (50 μM) was infiltrated with DMSO 18 h before sampling. The TAP tag gene was expressed as an internal control, and the protein was detected by immunoblot with the peroxidase antiperoxidase (PAP) antibody. The transcriptional level of each gene was determined by RT-PCR.