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Degradation of APIP6 by AvrPiz-t in N. benthamiana.
Agrobacterium carrying FLAG:APIP6 H58Y construct was coinfiltrated into N. benthamiana leaves with either an empty vector (ev), GFP:HA (G), or GFP:AvrPiz-t:HA (without the signal peptide sequence). The agroinfected tissues were harvested 3 d after treatment. MG132 (50 μM) was infiltrated with DMSO 18 h before sampling. The transcriptional level of each gene was determined by RT-PCR.
