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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Cancer Res. 2012 Dec 7;72(24):6382–6392. doi: 10.1158/0008-5472.CAN-12-1033

Figure 2.

Figure 2

TWIST1 is required and sufficient for melanoma cell invasion through Matrigel. (A) WM793 and (B) WM115 cells constitutively expressing control or one of three independent TWIST1 shRNAs were utilized. Cells were allowed to invade through Matrigel-coated chambers towards an attractant of full serum media. Counts taken (in triplicate fields of view) from the control shRNA (average set at 100% invasion) were used to calculate percent invasion for all other treatments. Columns, average of three independent experiments; bars, SD; scale, 100µm; **p<0.01. (C) WM3211 and (D) Sbcl2 cells overexpressing either LacZ (control) or TWIST1 were assessed for invasion through Matrigel-coated boyden chambers, as above. Columns, average of three independent experiments; bars, SD; scale, 100µm; **p<0.01.