Table 2.
List of modified peptides included in the targeted mass list
| Modified Peptidea | Modification Siteb | Typec |
|---|---|---|
| ALVLIAFAQYLQQC#PFEDHVK | Cys34 | MA |
| SLH@TLFGDK | His67 | MA |
| NECFLQH@K | His105 | MA |
| VH@TECCHGDLLECADDR | His242 | MA |
| VHTECCH@GDLLECADDR | His247 | MA |
| SH@CIAEVENDEM*PADLPSLAADFVESK | His288 | MA |
| SH@CIAEVENDEMPADLPSLAADFVESK | His288 | MA |
| CCAAADPH@ECYAK | His367 | MA |
| EFNAETFTFH@ADICTLSEK | His510 | MA |
| LVNEVTEFAKˆTCVAD | Lys51 | MA |
| YKˆAAFTECCQAADK | Lys162 | MA |
| LKˆCASLQK | Lys199 | MA |
| AFKˆAWAVAR | Lys212 | MA |
| AEFAEVSKˆLVTDLTK | Lys233 | MA |
| ADLAKˆYICENQDSISSK | Lys262 | MA |
| LAKˆTYETTLEK | Lys351 | MA |
| VFDEFKˆPLVEEPQNLIK | Lys378 | MA |
| KˆVPQVSTPTLVEVSR | Lys414 | MA |
| K∼VPQVSTPTLVEVSR | Lys414 | SB |
| KˆQTALVELVK | Lys525 | MA |
| K∼QTALVELVK | Lys525 | SB |
a
Unmodified versions of the listed modified peptides and peptides resulting from cleavage with trypsin of unmodified versions of the listed modified peptides were also targeted; C# indicates HNE Michael addition at Cys followed by reduction; H@ indicates HNE Michael addition at His followed by reduction; Kˆ indicates HNE Michael addition at Lys followed by reduction; K∼ indicates Schiff base formation with HNE at Lys followed by reduction; M* indicates oxidation at Met
b
Secreted protein numbering; add 24 for nascent protein
c
MA indicates Michael addition; SB indicates Schiff base formation