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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: ChemMedChem. 2012 May 23;7(7):1286–1294. doi: 10.1002/cmdc.201200104

Figure 2.

Figure 2

Biochemical characterization of 1 and analogues. (A) Time course of the polymerization of 30% pyrene-labeled actin in the presence of 5 nM Arp2/3 complex, 200 nM N-WASP-VCA, and either DMSO for a range of concentrations of 1. A control reaction (actin alone) lacking only Arp2/3 complex shows the intrinsic nucleation rate of actin. (B) Comparison of actin polymerization time courses in the presence of equal concentrations of 1 versus 2. Conditions are identical to panel (A). (C) Plot of maximal polymerization rate versus inhibitor concentration for 1, 2, and 5. Data were fit as described in Methods.