Fig. 2. Anti-DNMT1 immunohistochemistry in human intestinal type gastric cancer.
A. 10×; B. 40×. Almost all DNMT1 positive cells are seen in the tumor epithelium and not in the stroma. Thus while DNMT1 mRNA expression can be detected in the stromal cells by sensitive RT-PCR, the protein levels appear much reduced relative to the levels in the malignant epithelial cells. On serial sections the stromal cells were strongly αSMA positive (not shown).