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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Bone Miner Res. 2012 Jun;27(6):1263–1274. doi: 10.1002/jbmr.1574

Fig. 4.

Fig. 4

ERK1/2 MAP kinase activity is required for FFSS-induced RUNX2 phosphorylation, histone modifications, and gene expression. Cells were exposed to FFSS for 1 hour in the presence or absence of MAPK inhibitor, U0126 (10 μM), and were either immediately harvested for Western blot and ChIP analysis (B–D) or cultured for an additional 12 hours for mRNA detection (A). (A) Gene expression. The indicated mRNAs were measured by real-time RT-PCR and results were normalized to GAPDH mRNA. Significantly different from corresponding static control, ap <0.05, bp <0.01, n = 3. (B) ERK1/2 and RUNX2-S319 phosphorylation. (C,D) P-ERK binding to chromatin and histone modifications. ChIP assays were conducted as in Fig. 2 using primers to OSE2a and OSE2b regions of the mOG2 promoter.