Figure 1.

TLR4 stimulationPer triggers LRRK2 expression in microglia cells. Five micrograms of LPS (Escherichia coli 0111:B4) was unilaterally injected into the SNpc or striatum of 12-week-old male WT and LRRK2 KO C57BL/6J 12 mice. A–L, Immunohistochemistry for TH (A–C), isolectin B4 (marker for microglia and endothelial cells) (D–F), or LRRK2 was performed on serial coronal sections spanning the SNpc and striatum (G–L). Arrowheads indicate LRRK2 immunoreactivity on cells in the SNpc with the size and location of TH-positive neurons on both the contralateral and ipsilateral injection sides. Arrows indicate intense LRRK2 staining in numerous small cells observed exclusively on the ipsilateral side. WM, White matter; Str, striatum. No specific cellular staining in these areas was observed when primary antibodies were replaced with species-matched whole IgG (data not shown). Scale bar: A–L (in A), 50 μm.