(A) Wild-type (filled symbols) and trisomic primary (open symbols) MEFs were grown for 72 hours either in the absence (circles) or presence (0.2 mM, triangles; 0.5 mM, squares) of AICAR and cell number was determined at the indicated times.
(B) Cell number of wild-type (filled bars) and trisomic cells (open bars) was determined after 3 days and is shown as the percentage of the untreated control. The data in this and all subsequence figures are shown as the mean ± standard deviation. *P<0.05, **P<0.005, t test.