(A) Mice were implanted with 4 ×106 MIN cells on the left flank and with the same number of CIN cells on the right flank. Seven days after injection (indicated by the arrow) mice were treated with daily i.p. injections of AICAR, 17-AAG, both or PBS. Tumor volume (mm3) was measured at the indicated time points and shown as mean tumor volumes.
(B) Mice treated with PBS (left) or AICAR+17AAG (right) 25 days after transplantation.
(C) Quantification of the percentage of annexin V-FITC positive, PI negative cells in wild-type, MIN and CIN cell cultures 24 hours after AICAR or AICAR+17-AAG treatment. *P<0.05, **P<0.005, t test.