Table 2. Determination of NDV titer in the NDV-CS-NPs.
Ratio ofvirus dilution | Observational result | Accumulative result | ||||
Cytopathiceffect | No cytopathiceffect | Cytopathiceffect (%) | Cytopathiceffect | No cytopathiceffect | Cytopathiceffect (%) | |
10−6 | 4 | 1 | 80 | 5 | 1 | 83.3 |
10−7 | 1 | 4 | 20 | 1 | 5 | 16.7 |
10−8 | 0 | 5 | 0 | 0 | 10 | 0 |
The viral titer in the NDV-CS-NPs was 106.5 EID50/0.1 mL as calculated using the Reed-Muench method.