Figure 4.

IL-1β induces EMT in colon cancer cells. (A) IL-1β induces morphological changes from epithelial-like to fibroblast-like appearances in colon cancer cells. HCT-116 and HPCC cells were cultured in the medium with 1% FBS in the presence or absence of IL-β for seven days. Scale bar = 200 μm. (B) Transcription levels of Zeb1 and E-cadherin as determined by real-time PCR. β-actin was used as an internal normalization control. Error bars represent SEM. *p < 0.05. (C) Immunoblot analysis for E-cadherin from control and IL-1β-treated cells. β-actin was used as an sample loading control.