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Northern blot analysis of novel regions. For Northern blots, 12 μg of cytosolic RNA and the poly(A)+ fraction from each of the specified cell lines was loaded on the gel. The filters were hybridized with radiolabeled DNA probes corresponding to the cloned RT-PCR products derived from the novel array-predicted transcribed regions described in both the present and previous reports (Kapranov et al. 2002).
