Figure 8. R2HBJJ down-regulated the levels of cell cycle regulatory proteins and Rb phosphorylation.

H1299 cells were treated with 20 µM R2HBJJ for the indicated times (3–60 h, A) or with various concentrations of R2HBJJ (1.25–20 µM) for 48 h (B). Cell lysates were then subjected to western blot analysis. β-actin was used as the loading control.