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. 2012 Dec 28;7(12):e53214. doi: 10.1371/journal.pone.0053214

Figure 1. Gel analysis of F. rubra and E. festucae antisense transcripts.

Figure 1

The diagram illustrates primer design for detection of sense and antisense transcripts. The “A” primers were used for strand specific synthesis of cDNA from the RNA sample. The “A” and “B” primers were used for cDNA amplification. cDNAs generated from gene-specific primers for the F. rubra metallothionein (MT) and the E. festucae NC12, antifungal protein (AFP), and subtilisin-like protease were used as templates for PCR amplification.