Figure 8. Effects of pharmacological agents on NaCl stress-induced H⁺ flux across the plasma membrane.

P. euphratica cells were untreated (control) or treated with NaCl (200 mM) or NaCl plus ATP (200 µM) in the presence or absence of suramin (300 µM), PPADS (300 µM), or the H–G system (50 mM glucose and 100 units/mL hexokinase). (A) Transient H⁺ flux. SIET data are representative of six independent experiments. (B) Peak and mean values of H⁺ fluxes before (–) and after (+) the addition of NaCl or NaCl plus ATP. Bars represent the mean of six individual cells, and whiskers represent the standard error of the mean. N.S. = no significant difference.