Table 5.
Ratios of differentially expressed miRNAs in infarcted tissue using TaqMan based and Sybr Green approach
| |
TaqMan |
Sybr Green |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| miR-1 | miR-133a | miR-133b | miR-98 | miR-21 | miR-122 | miR-125a-5p | miR-125b | miR-126 | |
| FFPE RNU48 |
0.10 |
1.24 |
0.25 |
0.36 |
nd |
nd |
nd |
nd |
nd |
| RNAlater RNU48 |
0.47 |
0.46 |
0.39 |
0.62 |
nd |
nd |
nd |
nd |
nd |
| FFPE RNU6B |
0.11 |
0.68 |
0.29 |
0.41 |
0.59 |
1.71 |
0.49 |
0.44 |
0.29 |
| RNAlater RNU6B |
0.22 |
0.22 |
0.18 |
0.29 |
1.65 |
0.18 |
1.04 |
1.18 |
0.99 |
| FFPE miR-26b |
0.20 |
1.25 |
0.53 |
0.74 |
5.03 |
14.52 |
4.21 |
3.73 |
2.49 |
| RNAlater miR-26b |
0.78 |
0.77 |
0.64 |
1.02 |
2.64 |
0.29 |
1.67 |
1.90 |
1.59 |
| microarray results | 0.10 | 0.35 | 0.35 | 0.30 | 0.97 | 9.71 | 0.35 | 0.74 | 0.25 |
The ratios are calculated according to the efficiency corrected model [46]. Expression in remote myocardium is set at one, and expression in infarcted tissue is calculated as a proportion of the expression in corresponding remote myocardium. Further, three different genes were used as RGs. Legend: FFPE, formalin-fixed paraffin-embedded tissue; RNAlater, samples stored in RNAlater; RNU48, the small RNA RNU48 is used as RG; RNU6B, the small RNA RNU6B is used as RG; miR-26b, the miR-26b is used as RG.