Table 1.
miRNAs most preferentially released into EVs
|
miRNA |
Non-transfected cells & EVs |
miR-146a - Transfected cells & EVs |
||||
|---|---|---|---|---|---|---|
| RPMM cells | RPMM EV | Log2(EV/Cells) | RPMM cells | RPMM EV | Log2(EV/Cells) | |
|
mir-451 |
0.4 |
3945.0 |
13.3 |
1.7 |
7982.5 |
12.2 |
|
mir-150 |
0.4 |
2475.0 |
12.6 |
0.3 |
663.0 |
10.9 |
|
mir-146a* |
10.4 |
1321.2 |
7.0 |
317863.3* |
440496.3* |
0.5 |
|
mir-320c |
24.3 |
2347.5 |
6.6 |
57.8 |
1803.4 |
5.0 |
|
mir-143 |
39.9 |
3232.5 |
6.3 |
8327.3 |
354.4 |
8.5 |
|
mir-720 |
65.4 |
3157.5 |
5.6 |
3208.9 |
46.2 |
5.5 |
|
mir-125a-5p |
214.7 |
9360.0 |
5.4 |
439.0 |
2121.6 |
2.3 |
|
mir-486 |
105.7 |
3900.0 |
5.2 |
72.8 |
7637.8 |
6.7 |
|
mir-149 |
89.0 |
3142.5 |
5.1 |
85.3 |
928.2 |
3.4 |
|
mir-2110 |
43.5 |
1380.0 |
5.0 |
175.0 |
928.2 |
2.4 |
|
mir-320a |
762.1 |
23190.0 |
4.9 |
1250.0 |
11483.2 |
3.2 |
|
mir-197 |
472.4 |
8347.5 |
4.1 |
724.0 |
6046.6 |
3.1 |
|
mir-339 |
91.4 |
1515.0 |
4.1 |
178.0 |
610.0 |
1.8 |
|
mir-99b |
2065.2 |
27420.0 |
3.7 |
4900.0 |
8353.8 |
0.8 |
|
mir-4286 |
108.5 |
1245.0 |
3.5 |
72.1 |
1617.7 |
4.5 |
|
mir-92b |
515.1 |
5542.5 |
3.4 |
484.0 |
4800.1 |
3.3 |
|
mir-222 |
774.1 |
7882.5 |
3.3 |
1200.0 |
10634.5 |
3.1 |
|
mir-423-5p |
5737.2 |
55762.5 |
3.3 |
5290.0 |
22435.9 |
2.1 |
|
mir-877 |
467.2 |
4425.0 |
3.2 |
2000.0 |
2121.6 |
0.1 |
|
mir-191 |
9501.8 |
88717.5 |
3.2 |
15000.0 |
40893.8 |
1.4 |
|
mir-1292 |
140.0 |
1222.5 |
3.1 |
256.0 |
397.8 |
0.6 |
| mir-1246 | 782.8 | 6570.0 | 3.1 | 156.0 | 11456.6 | 6.2 |
Those miRNAs represented by at least 1000 reads in either cells or EV are listed. * For the transfected cells and EVs the RPMM values were calculated after removal of the reads from over-expressed miR-146a, to facilitate comparison with non-transfected samples (the RPMM values shown for miR-146a are those prior to this normalisation).