Figure 7. Alternative transcripts in DNA repair genes are induced by IR.

Alternative transcripts in the DNA repair genes, XPC (A) and RRM2B (B) in response to IR are shown. Graph axes are as in figure 1; PCR products from 5′-RML-RACE were run on a 2% agarose gel. An arrow indicates the amplicon from the alternative initiated transcript that was sequenced (gel picture to the right of the panel; Figure S4). Diagrams of the predominant transcripts (initiating by P1) and the alternatively initiated (P2) transcripts after IR are shown below. Primer locations for 5′ RLM-RACE are indicated below exon 2 (arrow pointing to the left).