Figure 5. Purified monocytes and M-CSF treated (10 ng/ml) purified monocytes from the same donor were incubated with CRP (200 µg/ml) for 24 h and supernatants were analyzed for pro-inflammatory and anti-inflammatory cytokines.

Control values were several thousand-fold less than treated values. Monocytes secreted pro-inflammatory cytokines in response to CRP, which decreased after M-CSF differentiation. The differentiated monocytes secreted anti-inflammatory cytokines, IL-1RA and IL-10. *, P<0.05 vs. purified monocytes; nonparametric Mann Whitney test was used to analyze differences between groups. Mean ±SEM of triplicate wells from 3 independent experiments.