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. 2012 Nov 21;161(1):121–133. doi: 10.1104/pp.112.210914

Figure 3.

Figure 3.

Vacuolar proteins accumulate in the ER in vsr1vsr4 double mutant plants. A, Subcellular localization of AALP:GFP in the vsr1vsr4 double mutant protoplasts. Wild-type protoplasts were transformed with AALP:GFP and vsr1vsr4 mutant protoplasts were cotransformed with AALP:GFP and BiP:mRFP. At 24 h after transformation, localization of these proteins was examined by fluorescence microscopy. CH, chloroplast autofluorescence. Bar = 20 µm. B, Subcellular localization of phaseolin in the vsr1vsr4 double mutant protoplasts. Wild-type protoplasts were transformed with phaseolin and vsr1vsr4 protoplasts were cotransformed with phaseolin and BiP:mRFP. Localization of phaseolin was determined by immunostaining with an antiphaseolin antibody followed by an FITC-labeled secondary antibody. BiP:mRFP was observed directly. Bar = 20 µm.