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. 2012 Nov 21;161(1):121–133. doi: 10.1104/pp.112.210914

Figure 4.

Figure 4.

Vacuolar trafficking of AtβFruct4:GFP is not inhibited in vsr mutant plants. A, Western-blot analysis of AtβFruc4t:GFP trafficking in vsr1vsr3, vsr1vsr4, and vsr5vsr6 double mutants. Protoplasts from wild-type and indicated vsr mutant plants were transformed with AtβFruct4:GFP. At 24 h after transformation, proteins extracted from protoplasts were analyzed by western blotting using anti-GFP antibody. Targeting efficiency was expressed as a relative value of the amount of precursor (Pre) and processed protein (Pro) to the amount of total proteins. Error bars indicate sd (n = 3). Col, Col-0 wild type; 1/3, vsr1vsr3 mutant; 1/4, vsr1vsr4 mutant; 5/6, vsr5vsr6 mutant. B, Subcellular localization of AtβFruct4:GFP in vsr1vsr4 mutant protoplasts. Protoplasts derived from the wild type and vsr1vsr4 were transformed with AtβFruct4:GFP. Localization of AtβFruct4:GFP was examined under a fluorescent microscope. WT, Col-0 wild-type. Bars = 20 μm.