Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Nov 21;161(1):121–133. doi: 10.1104/pp.112.210914

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 American Society of Plant Biologists. All Rights Reserved.

PMC Copyright notice

Figure 6. — Ectopic expression of VSR5:HA increases the secretion of cargo proteins in vsr1vsr4 double mutant protoplasts. A and B, Effect of VSR5:HA expression on the trafficking of AALP:GFP and phaseolin in the vsr1vsr4 double mutant. Protoplasts derived from vsr1vsr4 mutant plants were cotransformed with the indicated amount of VSR5:HA together with either AALP:GFP (A) or phaseolin (B). Protein extracts from transformed protoplasts (P) and incubation medium (S) were analyzed by western blotting using anti-GFP or antiphaseolin antibody. Expression of VSR5:HA was detected by anti-HA antibody. Actin was detected as a control for protein leakage from protoplasts and as a loading control. Pre, precursor form; Pro, processed form.