Figure 3.

Expression patterns of GUS driven by the CnAIP2 promoter at different stages during the Arabidopsis life cycle. A, Developing and mature seeds were stained with X-gluc directly or incubated in one-half-strength MS liquid medium with 20 µm 2,4-D overnight before X-gluc staining. Seed coats of seeds older than 7 DAP were removed before incubating in X-gluc or 2,4-D solution. B, Seedling and flower tissues were collected and directly stained with X-gluc without any treatment. Chlorophyll in green tissues was removed by incubating samples in 95% ethanol overnight. Photographs are cropped at different magnifications to show proper details.