Seed development of some CnAIP2-expressing lines was impaired, leading to abortion at early stages. A, An example of siliques expressing CnAIP2 with impaired seed development, in comparison with wild-type (WT) siliques as a control. All siliques were collected 2 weeks after pollination. B, CnAIP2 protein levels in 2-week-old siliques. Lines 1, 2, 4, 8, 9, and 10 were unable to produce seeds; lines 3 and 5 produced small siliques with a few seeds; lines 6, 7, and 11 produced normal seeds. The arrow indicates the CnAIP2 protein. [See online article for color version of this figure.]