Figure 5.

Germination characteristics and ABI3 expression levels of wild-type (WT) and CnAIP2 transgenic seeds. A, Germination performance of seeds either without (0d-C) or with (1d-C) a 1-d moist-chilling treatment prior to transfer of seeds to germination conditions. B, RT-PCR transcript analysis to detect ABI3 expression levels in seeds under germination conditions without a prior moist-chilling treatment. The housekeeping gene EF1-α was used as the control. Numbers above the gels indicate days in germination conditions on one-half-strength MS plates. C, Germination performance of seeds in the presence of ABA. Seeds were subjected to a 4-d moist-chilling treatment (4°C) prior to transfer to germination conditions. Plates contained one-half-strength MS medium with either 0.5 or 1.0 µm (+)-ABA. On plates without ABA, all seeds germinated 1 d after their transfer to germination conditions. Data represent means ± se of duplicate tests, each with 50 to 100 seeds.