Figure 2.

CIPK9 interaction with CBL2 and CBL3. A, Interaction analysis of CIPK9 with all Arabidopsis CBLs using yeast two-hybrid assays. B, Quantitative analysis of β-galactosidase activity of the positive clones containing the CIPK9/CBL complex. Data shown are means ± se (n = 3). U, Units. C, Interaction analysis of CIPK9 with CBL2 and CBL3 using pull-down assays. The GST-CBL2 and GST-CBL3 fusion proteins were used as bait to pull down from the whole plant proteins that were extracted from cMyc-CIPK9-transformed plants (cMyc-CIPK9) or wild-type plants (Col). The GST protein was used as a negative control. The top panel shows the western blot probed with anti-cMyc antibody. The arrow indicates the site of cMyc-CIPK9 protein. The bottom panel shows protein gel analysis indicating the amount of bait proteins used in pull-down assays.