FISH-based characterization of Tnt1 mutant lines BS5-6 and BS6-19. A and C, Chromosomes of line BS5-6 or the parent line cv Maverick were hybridized with Texas Red-labeled pSH-Tnt1 plasmid DNA, respectively. E and G, Chromosomes of line BS5-6 or cv Maverick were hybridized with Texas Red-labeled pBS-Tnt1 plasmid DNA, respectively. I, Chromosomes of line BS6-19 were hybridized with pBS-Tnt1 probe. B, D, F, H, and J, Grayscale images of the chromosomes in A, C, E, G, and I, respectively. K, Fiber-FISH shows an approximately 34-kb Tnt1 DNA fiber in line BS6-19.