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Southern-blot analysis of the Tnt1 line BS5-12-8R and its T1 progeny obtained by self-pollination. Fifteen micrograms of chromosomal DNA from different plants was digested with NdeI and hybridized with Tnt1 probe. M, Molecular weight markers; T0, BS5-12-8R; T1, progeny of BS5-12-8R.
