Figure 6.

Remobilization of Tnt1 transposition by tissue culture using cotyledons as explants with wound or wounding plus Suc treatment. Southern-blot analysis was performed by using NdeI-digested chromosomal DNA of regenerated plants from Tnt1 lines BS5-14 and BS5-12 with the Tnt1 probe. The hybridization bands that presented in parent lines are marked with asterisks, and the unmarked bands were potential novel insertions in regenerated plants. M, molecular weight standards; P1 and P2, Tnt1 primary transgenic lines BS5-14 and BS5-12, respectively; RA1, plants regenerated from line BS5-14; RA2, plants regenerated from line BS5-12; W, plants regenerated from wound-treated cotyledons; S, plants regenerated from wound plus 1 m Suc-treated explants.