Table II. Segregation of Tnt1 insertions in line BS5-12-8R.
PCR analysis results are shown for the segregation of Tnt1 insertions in the progeny of line BS5-12-8R. Insertions 1, 2, 3, and 4 were inserted into Glyma20g34300, Glyma17g35300, Glyma01g01300, and Glyma19g07410, respectively. +/+, Wild-type homozygous plants; +/−, heterozygous plants; −/−, homozygous plants for a given Tnt1 insertion.
| BS5-12-8R | Insertion No. 1 | Insertion No. 2 | Insertion No. 3 | Insertion No. 4 |
|---|---|---|---|---|
| T0 | +/− | +/− | +/− | +/− |
| Plant 1 | −/− | +/− | +/− | +/− |
| Plant 2 | +/− | +/− | +/+ | +/− |
| Plant 3 | +/− | +/+ | +/− | +/+ |
| Plant 4 | +/− | +/+ | +/− | +/− |
| Plant 5 | +/− | +/− | +/− | −/− |
| Plant 6 | +/− | +/− | +/+ | +/− |
| Plant 7 | +/− | −/− | +/− | +/− |
| Plant 8 | −/− | +/+ | +/− | +/− |
| Plant 9 | −/− | +/− | +/− | +/− |
| Plant 10 | +/+ | +/+ | +/− | +/− |
| Plant 11 | +/− | +/− | +/− | +/− |
| Plant 12 | +/+ | +/− | +/− | −/− |
| Plant 13 | +/− | +/− | +/+ | +/− |
| Plant 14 | +/− | +/− | +/− | +/+ |
| Plant 15 | +/− | +/+ | +/− | +/− |
| Plant 16 | +/− | +/− | +/− | +/− |
| Plant 17 | −/− | −/− | +/− | +/− |
| Plant 18 | +/+ | +/+ | +/− | +/− |
| cv Maverick | +/+ | +/+ | +/+ | +/+ |
| Ratio | ||||
| +/+ | 3 | 6 | 3 | 2 |
| +/− | 11 | 10 | 15 | 14 |
| −/− | 4 | 2 | 0 | 2 |