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. 2012 Nov 12;161(1):374–383. doi: 10.1104/pp.112.209213

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Figure 2. — In vivo pull-down assay for the interaction between ToMV MP and NbRbCS. Immunoprecipitation (IP) was performed on extracts containing transiently expressed 3×HA-NbRbCS and ToMV MP-4×Myc or extracts containing transiently expressed ToMV MP-4×Myc and 3×HA by using protein A/G agarose beads incubated with anti-HA antibody. After immunoprecipitation, precipitates were analyzed by western blot using anti-Myc (top panel) or anti-HA (middle panel) antibody. The expression of ToMV MP-4×Myc was confirmed by western-blot analysis with anti-Myc antibody (bottom panel). IB, Immunoblot.