Figure 5.

Effect of A. tumefaciens-mediated transient expression of Pto, avrPto, Pto/avrPto, and CaXEGIP1 on the cell death response of N. benthamiana leaves. A and B, RT-PCR (A) and immunoblot (B) analyses of CaXEGIP1 expression in N. benthamiana leaves. Expression of the N. benthamiana Actin gene served as a control. Protein loading was verified by Coomassie Brilliant Blue (CBB) staining. C, Induction of cell death in N. benthamiana leaves. D, Measurements of electrolyte leakage from N. benthamiana leaves infiltrated with A. tumefaciens (OD₆₀₀ = 0.5) carrying the indicated constructs. The blue, yellow, and red circles indicate no cell death, intermediate cell death, and full cell death, respectively. The data represent means ± sd from three independent experiments. Different letters indicate statistically significant differences, as determined by Fisher’s lsd test (P < 0.05).