Table II. Carboxylic acid content of fruit of transgenic plants expressing an antisense aconitase construct.
Fruit extracts were analyzed using GC-MS. Tomatoes were harvested at 40 and 55 DAA as indicated. Data are means ± se of six independent fruits. Values in boldface are significantly different from the wild type (Student’s t test, P < 0.05).
| Metabolite Level in Fruit (Relative to the Wild Type) |
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|---|---|---|---|---|---|---|
| Metabolite | 40 DAA |
55 DAA |
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| Wild Type | ACO19 | ACO38 | Wild Type | ACO19 | ACO38 | |
| Citrate | 1.00 ± 0.04 | 0.84 ± 0.06 | 0.70 ± 0.08 | 1.00 ± 0.03 | 1.38 ± 0.05 | 1.42 ± 0.05 |
| Succinate | 1.00 ± 0.02 | 1.12 ± 0.02 | 1.17 ± 0.03 | 1.00 ± 0.02 | 0.81 ± 0.00 | 0.80 ± 0.00 |
| Fumarate | 1.00 ± 0.08 | 1.21 ± 0.07 | 1.01 ± 0.02 | 1.00 ± 0.03 | 0.62 ± 0.02 | 0.64 ± 0.02 |
| Malate | 1.00 ± 0.08 | 0.79 ± 0.04 | 0.98 ± 0.10 | 1.00 ± 0.10 | 1.63 ± 0.24 | 1.89 ± 0.14 |