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PNGase F-resistant glycans with terminal GlcNAc on fucta/fuctb/xylt triple mutant proteins are resistant to β-elimination chemistry. Glycans with terminal GlcNAc on a blot containing PNGase F-treated fucta/fuctb/xylt triple mutant proteins were detected by labeling with [3H]Gal. The lane on the left shows the blot prior to β-elimination, and the right lane (β-elm.) shows the same blot after subjecting it to β-elimination chemistry, which removes O-linked glycans.
