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. 2012 Nov 8;161(1):455–464. doi: 10.1104/pp.112.208900

Figure 4.

Figure 4.

TGG1 and TGG2 from wild-type plants are N-GlcNAc modified. A, Immunoblot detection of TGG1 and TGG2 from wild-type (WT) and triple mutant plants following treatment with or without PNGase F. PNGase F treatment removes high-Man glycans, which increases the rate of TGG1 and TGG2 migration during electrophoresis (Ueda et al., 2006). B, Immunoblots showing the effect of PEGylating wild-type and triple mutant proteins after treatment with or without PNGase F. Asterisks indicate the PEGylated proteins.