Figure 2.

A and B, Absorption spectra of whole cells of C. reinhardtii at room temperature (A) and fluorescence emission spectrum at 77 K upon excitation at 460 nm (B). Solid lines, the wild type; dashed lines, Fud7-P71 strain; dashed-dotted lines, FN68. The spectra are normalized to maximal absorption and fluorescence emission, respectively. a.u., Arbitrary units. C, Deriphat-PAGE of thylakoid membranes (35,000g fractions) from the wild type (WT) and FN68. PSI/PSII-sc, Nondissociated PSI/PSII supercomplexes; LHC-m, monomeric LHC; FP, free pigment. Lanes were loaded based on equal Chl (5 µg Chl a+b) concentration. D, Immunoblot analysis of whole cell extracts using antibodies raised to subunits of PSI (PsaD, PsaF, PsaN), PSII (D1), Cyt b₆f complex (Cyt. f), and LHC of PSI (p11) and of PSII (p15). Lanes were loaded based on equal cell numbers. [See online article for color version of this figure.]