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. 2012 Nov 7;161(1):556–567. doi: 10.1104/pp.112.206110

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Figure 6. — Nod factor-induced calcium flux is absent in the 5′D LNP antisense line. Calcium levels were monitored in individual root hairs of wild-type, vector control, and 5′D LNP antisense plants after the addition of 100 nm Nod factor (black vertical line). Ratios (arbitrary units) of the fluorescence of Oregon Green (OG; calcium-sensitive dye) and Texas Red (TX; calcium-insensitive dye) were calculated every 5 s for more than 30 min. The number of cells showing calcium influx is shown as a fraction of the total number of cells analyzed (with the total number of plants tested in parentheses).