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UV/visible spectra of purified Cr-rPFO. The spectra were recorded in an anaerobic cuvette containing 10 µm enzyme in 100 mm Gly buffer at pH 10.5 in order to ensure full reduction by dithionite. Solid line, Cr-rPFO as purified (Ox); dotted line, Cr-rPFO after reduction by dithionite (Dith).
