Table III. Effect of diverse constituents of the assay on Cr-rPFO decarboxylase activity.

The complete reaction mixture was as in Table I using 1 mm BV as electron acceptor.

Reaction Mixture	Pyruvate Oxidation
	%
Complete	100
−CoA	0
−Pyruvate	0
−TPP	2.5
−MgCl2	56.6
+DTT (2.5 mm)	100
+NaCl (300 mm)	18
+Imidazole (30 mm)	100
+Cr-rPFO + air for 1 h at 4°C	17.4