Table 2.
Growth Inhibition verification of 2B/10A Compounds
| Chemical | CMap (μM) | GI50 (μM) | Subcomponent | Cell line |
|---|---|---|---|---|
|
berberine |
10 |
25.1 |
2B |
MCF7 |
|
irinotecan |
100 |
6.3 |
2B, 10A |
MCF7 |
|
mitoxantrone |
7.9 |
0.004 |
2B |
MCF7 |
|
amiodarone |
6.3 |
4.0* |
2B |
PC3 |
|
8-azaguanine |
25.1 |
0.32 |
10A |
HL60 |
|
apigenin |
15.9 |
25.1 |
10A |
HL60 |
|
azacitidine |
15.9 |
0.79 |
10A |
PC3 |
|
camptothecin |
12.6 |
<0.01** |
10A |
MCF7 |
|
chrysin |
15.9 |
15.8*** |
10A |
MCF7 |
|
methotrexate |
7.9 |
0.03 |
10A |
MCF7 |
|
thioguanosine |
12.6 |
0.32 |
10A |
MCF7 |
| esculetin |
25.1 |
>100** |
10A |
HL60 |
| fulvestrant | 1.0 | >100** | 10A | PC3 |
GI50 values (drug concentration causing a 50% growth inhibition) from NCI/DTP are shown along with the corresponding concentrations used in the Connectivity Map (CMap) data. By comparing the GI50 and CMap values we can get an idea of expected cell killing effect of the drug in the CMap data. Drugs that are expected to eventually kill the cells are shown in bold. GI50 and CMap concentration values are in μM scales.
* GI50 value at the end of the tested range.
** Mean of GI50 values from HL60 and MCF7 cell lines.
*** Value from HL60 cell line.