Table 4.

PCR primer sequence for candidate QTL-related gene and SNP type used for genotypic analysis

Candidate genes	Primer sequence (5'→3')	SNP
LpEUI	f: ACG TAC CTG TAC TGG CTG	C/G
	r: TTG CAG TTG TCC ACC ACG AA
LpPDT	f: GCA GAA CAA AAA CTC CAA GA	n.a. *a
	r: TTG GAT CAG CCA TAG ACG CC
LpGA3ox2	f: CGC GCT ACT TCG ACT TCC	n.a. *b
	r: GAA GAA GGA GGA GAT GGG C
LpDGL1	f: GTT AAC ATT GAT GAA GTT GC	A/G
	r: ACA CTC TTC TGG ACC TTG GC
LpGA20	f: GGG TGT ACC AGG AGT ACT G	n.a. *a
	r: TTA CCA TGA AGG TGT CGC CG
LpPh1	f: GCA TTA ATG ATG AAT GGG CT	A/G
	r: CAT CCA CAC CAG TTA TTC TC
LpPi37	f: CCA GCG GAT ATG CGC AAT CT	C/T
	r: CAA ATG CTC TCG GCT GAA GG
LpPIPK1	f: GGC CCT TGT AAA TAG TCT CC	G/T
	r: CCC TTG ACT GTA ATT GGC TC
LpPib	f: TCA CGG ATG AGA TCA TGG AC	A/T
	r: CTG AAG AAG TGT GAT GGA CT
LpD3	f: CCA AGA TGA AAT TGG ACC TC	A/G
	r: CTG CAT GTC CCG CAA GTT TG
*a no SNP was identified in PCR amplicon.
*b PCR amplicons were not obtained.