Table 1.
List of all the aminoacyl tRNA synthetases and their associated proteins, aaRS paralogs and editing domains with their CDD domain assignments and subcellular localization
| Gene name | Length (aa) | HMM based function assignment | E-value# | Swissprot function assignment | Subcellular location (PSORT II) | CDD based domain assignment | |
|---|---|---|---|---|---|---|---|
| CLASS I aaRS |
LmjF.12.0250 |
784 |
Cysteinyl-tRNA synthetase |
1.00E-191 |
Cysteinyl-tRNA synthetase |
Cytosol |
CysRS_Core; anticodon_la_like_superfamily |
| LmjF.15.1440 |
570 |
Glutaminyl-tRNA synthetase |
8.70E-208 |
Glutaminyl-tRNA synthetase |
Cytosol |
GlnRS_core; GlnRS_core; tRNA_synt_Ic_C_superfamily |
|
| LmjF.30.3240 |
594 |
Glutaminyl-tRNA synthetase |
7.00E-079 |
Glutaminyl-tRNA synthetase |
Cytosol |
GlnRS_core; GlnRS_core; tRNA_synt_Ic_C_superfamily |
|
| LmjF.27.1310 |
692 |
Arginyl-tRNA synthetase |
2.00E-151 |
Arginyl-tRNA synthetase |
Cytosol |
Arg_tRNA_synt_N; ArgRS_Core; anticodon_Ia_like_superfamily |
|
| LmjF.36.5620 |
1009 |
Isoleucyl-tRNA synthetase |
0 |
Isoleucyl-tRNA synthetase |
Cytosol |
IleRS_Core; Anticodon_Ia_like_superfamily |
|
| LmjF.13.1100 |
1075 |
Leucyl-tRNA synthetase |
3.70E-140 |
Leucyl-tRNA synthetase |
Cytosol |
IleRS_Core; Anticodon_Ia_like_superfamily |
|
| LmjF.21.0810* |
747 |
Methionyl-tRNA synthetase |
6.00E-145 |
Methionyl-tRNA synthetase |
Cytosol |
MetRS_Core_superfamily; Anticodon_Ia_Met |
|
| LmjF.23.0300 |
412 |
Tryptophanyl-tRNA synthetase |
1.90E-062 |
Tryptophanyl-tRNA synthetase |
Cytosol |
TrpRS_core |
|
| LmjF.29.0060 |
480 |
Tryptophanyl-tRNA synthetase |
1.70E-095 |
Tryptophanyl-tRNA synthetase |
Mitochondrial |
TrpRS_core |
|
| LmjF.14.1370* |
682 |
Tyrosyl-tRNA synthetase |
1.10E-051 |
Tyrosyl-tRNA synthetase |
Endoplasmic reticulum |
TyrRS_core; TyrRS_core |
|
| LmjF.30.3130 |
967 |
Valyl-tRNA synthetase |
0 |
Valyl-tRNA synthetase |
Cytosol |
ValRS_core; anticodon_Ia_Val; Val_tRNA_synt_C_superfamily |
|
| CLASS II aaRS |
LmjF.22.1540 |
962 |
Alanyl-tRNA synthetase |
3.10E-262 |
Alanyl-tRNA synthetase |
Cytosol |
AlaRS_core; tRNA_SAD |
| LmjF.30.0460 |
550 |
Aspartyl-tRNA synthetase |
3.90E-106 |
Aspartyl-tRNA synthetase |
Cytosol |
AspRS_cyto_N; AsxRS_Core |
|
| LmjF.21.0895 |
641 |
Aspartyl-tRNA synthetase |
5.70E-097 |
Aspartyl-tRNA synthetase |
Mitochondrial |
AspRS_cyto_N; ClassII_aaRS_like_core_superfamily; ClassII_aaRS_like_core_superfamily |
|
| LmjF.34.2340 |
890 |
Asparaginyl-tRNA synthetase |
2.40E-144 |
Asparaginyl-tRNA synthetase |
Cytosol |
tRNA_synt_Ic_R1_superfamily; AsnRS_cyto_like_N; AsxRS_core |
|
| LmjF.36.3840 |
628 |
Glycyl-tRNA synthetase |
5.10E-056 |
Glycyl-tRNA synthetase |
Cytosol |
GlyRS_core; HGTP_anticodon |
|
| LmjF.30.0130 |
586 |
Lysyl-tRNA synthetase |
8.20E-184 |
Lysyl-tRNA synthetase |
Cytosol |
LysRS_N; LysRS_Core |
|
| LmjF.15.0230 |
536 |
Lysyl-tRNA synthetase |
9.30E-148 |
Lysyl-tRNA synthetase |
Cytosol |
LysRS_N; LysRS_Core |
|
| LmjF.19.1040 |
633 |
Phenylalanyl-tRNA synthetase |
3.20E-053 |
Phenylalanyl-tRNA synthetase |
Cytosol |
B3_4_superfamily; B5_superfamily; PheRS_beta_core |
|
| LmjF.32.0870 |
499 |
Phenylalanyl-tRNA synthetase |
5.90E-052 |
Phenylalanyl-tRNA synthetase |
Cytosol |
PheRS_alpha_core |
|
| LmjF.18.1210 |
731 |
Prolyl-tRNA synthetase |
1.30E-188 |
Prolyl-tRNA synthetase |
Cytosol |
Ybak_like_superfamily; ProRS_arch_euk; HGTP_anticodon_superfamily |
|
| LmjF.18.1220 |
731 |
Prolyl-tRNA synthetase |
1.20E-190 |
Prolyl-tRNA synthetase |
Cytosol |
Ybak_like_superfamily; ProRS_arch_euk; HGTP_anticodon_superfamily |
|
| LmjF.11.0100 |
474 |
Seryl-tRNA synthetase |
4.40E-135 |
Seryl-tRNA synthetase |
Cytosol |
Seryl_tRNA_N_superfamily; SerRS_Core |
|
| LmjF.30.0630 |
473 |
Histidyl-tRNA synthetase |
2.30E-103 |
Histidyl-tRNA synthetase |
Cytosol |
HisRS_Core; HisRS_anticodon |
|
| LmjF.35.1410 |
787 |
Threonyl-tRNA synthetase |
3.20E-252 |
Threonyl-tRNA synthetase |
Cytosol |
TGS_superfamily; tRNA_SAD; ThrRS_Core; HGTP_anticodon |
|
| Non-canonical |
LmjF.09.0950 |
595 |
O-Phosphoseryl-tRNA Selenium transferase |
9.10E-127 |
SLA/LP autoantigen-like protein |
Nuclear |
Selenium_SpcS |
| aaRS associated proteins |
LmjF.22.0470 |
426 |
EMAP-II like |
1.20E-023 |
Hypothetical, Conserved |
Extracellular |
tRNA_binding_domain_superfamily |
| LmjF.16.1130 |
180 |
EMAP-II like |
1.20E-015 |
Tyrosyl/methionyl-tRNA synthetase |
Cytosol |
tRNAbindingdomain_superfamily |
|
| AsnRS paralog |
LmjF.26.0830 |
353 |
Asparagine Synthetase A |
2.00E-002 |
Aspartate, ammonia ligase |
Mitochondrial |
ClassII_aaRS_like_core_superfamily |
| Trans Editing domains | LmjF.15.0690 |
491 |
AlaX |
3.90E-028 |
Hypothetical, conserved |
Cytosol |
tRNA_SAD_superfamily |
| LmjF.03.0710 |
253 |
YbaK |
2.20E-002 |
Hypothetical, conserved |
Cytosol |
Ybak_like_superfamily |
|
| LmjF.21.0910 |
275 |
YbaK |
7.20E-003 |
Hypothetical, conserved |
Cytosol |
Ybak_like_superfamily |
|
| LmjF.36.2730 |
152 |
Dtda |
1.00E-026 |
D-tyrosine deacylase |
Cytosol |
Dtyr_deacylase |
|
| LmjF.34.3360* | 211 | Dtda | 5.40E-020 | Hypothetical, conserved | Cytosol | Dtyr_deacylase_superfamily |
* indicates L. major sequences whose crystal structures are available # E-value (Expectation value) is an indication of the significance of a hit to the HMM Model queried. This gives a more quantitative measure of statistical significance. The lower the E-value, the better is the significance of the hit to the query HMM.