Table 1. Binding constants of human SUV39H1 chromodomains and H3 histone peptides determined by fluorescence polarization.
Peptides | Kd (µM) for SUV39H1 (42–100) | Kd (µM) for SUV39H1 (44–106) |
H31–15K9me3 | 20±4 | *NB |
H31–15K9me2 | 29±12 | *NB |
H31–15K9me1 | *NB | *NB |
H31–15K9me0 | *NB | *NB |
NB: No detectable binding from FP experiments.